منابع مشابه
Enhanced Vibrational Spectroscopies as Tools for Small Molecule Biosensing
In this short summary we summarize some of the latest developments in vibrational spectroscopic tools applied for the sensing of (small) molecules and biomolecules in a label-free mode of operation. We first introduce various concepts for the enhancement of InfraRed spectroscopic techniques, including the principles of Attenuated Total Reflection InfraRed (ATR-IR), (phase-modulated) InfraRed Re...
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Microand nanofabrication has allowed the production of ultra-sensitive, portable, and inexpensive biosensors. These devices generally rely on chemical or biological receptors which recognize a particular compound of interest and relay this recognition event effectively by transduction. Recent advances in RNA and DNA synthesis have enabled the use of aptamers, in vitro generated oligonucleotides...
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Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.
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1.1. Macroscopic versus microscopic measurements in biology Macroscopic experiments yield time and population averages of the individual characteristics of each molecule. At the level of the individual molecules, the picture is quite different: individual molecules are found in states far from the mean population, and their instantaneous dynamics are seemingly random. Whenever unusual states or...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2016.11.422